CCMB-CSIR logo Structural Biology

Centre for Cellular and Molecular Biology
Hyderabad,INDIA
CSIR-CCMB
Sankar's Lab

 

Selected Publications
  1. Kumar P, Babu KSD, Singh AK, Singh DK, Nalli A, Mukul SJ, Roy A, Mazeed M, Raman B, Kruparani SP, Siddiqi I, Sankaranarayanan R. (2023) Distinct localization of chiral proofreaders resolves organellar translation conflict in plants. Proc Natl Acad Sci U S A (2023) doi: 10.1073/pnas.2219292120.

  2. Sivakumar K, Venkadasamy VL, Amudhan G, Ann KJ, Goud GK, Nayani K, Gogoi J, Kuncha SK, Mainkar PS, Kruparani SP, Sankaranarayanan R. (2023) Design principles and functional basis of enantioselectivity of alanyl-tRNA synthetase and a chiral proofreader during protein biosynthesis. Nucleic Acids Res. doi: 10.1093/nar/gkad205.

  3. Mondal,S., Kinatukara,P., Singh, S., Shambhavi, S., Patil,G.S., Dubey,N., Singh,S.H., Pal,B., Shekar,P.C., Kamat,S.S., Sankaranarayanan, R. (2022) DIP2 is a unique regulator of diacylglycerol lipid homeostasis in eukaryotes.  elife doi: 10.7554/eLife.77665.

  4. Kumar, P., Bhatnagar, A. & Sankaranarayanan, R. (2022) Chiral Proofreading during protein biosynthesis and its evolutionary implications. FEBS Lett DOI: 10.1002/1873-3468.14419.

  5. Gogoi, J., Bhatnagar, A., Ann, K.J., Pottabathini, S., Singh, R., Mazeed, M., Kuncha, S.K., Kruparani, S.P., Sankaranarayanan, R. (2022) Switching a conflicted bacterial DTD-tRNA code is essential for the emergence of mitochondria. Sci. Adv. 8, eabj7307, 1-9. doi: 10.1126/sciadv.abj7307.

  6. Patil G. S. , Kinatukara P., Mondal S., Shambhavi S., Patel K.D., Pramanik S., Dubey N., Narasimhan S., Madduri M K, Pal B, Gokhale R S, & Sankaranarayanan R. (2021)A universal pocket in fatty acyl-AMP ligases ensures redirection of fatty acid pool away from coenzyme A-based activation.   elife doi: 10.7554/eLife.70067.

  7. Mazeed, M. , Singh, R., Kumar P., Roy, A., Raman, B., Kruparani , S.P., & Sankaranarayanan, R. (2021) Recruitment of Archaeal DTD is a Key Event Toward the Emergence of Land Plants. Sci. Adv. 2021; 7 : eabe8890 DOI: 10.1126/sciadv.abe8890

  8. Kuncha, S. K., Venkadasamy, V. L., Amudhan, G., Dahate, P., Kola, S.R., Pottabathini, S., Kruparani , S.P., Shekar, P.C. , & Sankaranarayanan, R. (2020) Genomic innovation of ATD alleviates mistranslation associated with multicellularity in Animalia. eLife 2020;9:e58118.
    (Covered by elife Science Digest- June 18 2020: 'Proofreading to evolve' )

  9. Kuncha, S. K., Kruparani, S. P. and Sankaranarayanan, R. (2019) Chiral checkpoints during protein biosynthesis. J Biol Chem doi: 10.1074/jbc.REV119.008166.

  10. Routh S.B. & Sankaranarayanan R. (2018) Enzyme action at RNA-protein interface in DTD-like fold.  Curr Opin Struct Biol. 53, 107-114. doi: 10.1016/j.sbi.2018.07.013. [Epub ahead of print] Review.

  11. Kuncha S.K., Suma K., Pawar K.I., Gogoi. J, Routh S.B., Pottabathini S., Kruparani S.P. & Sankaranarayanan R. (2018) A discriminator code–based DTD surveillance ensures faithful glycine delivery for protein biosynthesis in bacteria.   elife doi: 10.7554/eLife.38232.

  12. Kuncha S.K.,Mazeed M., Singh R., Kattula B., Routh S.B. & Sankaranarayanan R.(2018) A chiral selectivity relaxed paralog of DTD for proofreading tRNA mischarging in Animalia.   Nature Commun. 9:511 DOI: 10.1038/s41467-017-02204-w

  13. Pawar, K.I., Suma, K., Seenivasan, A., Kuncha, S.K., Routh, S.B., Kruparani, S.P., Sankaranarayanan, R. (2017) Role of D-aminoacyl-tRNA deacylase beyond chiral proofreading as a cellular defense against glycine mischarging by AlaRS. elife doi: 10.7554/eLife.24001.
    (Covered by a Spotlight article in TIBS, September 2017, vol. 42; p684)

  14. Routh, S. B., Pawar, K. I., Ahmad, S., Singh, S., Suma, K., Kumar, M., Kuncha, S. K., Yadav, K., Kruparani, S. P. and Sankaranarayanan, R. (2016) Elongation factor Tu prevents misediting of Gly-tRNA(Gly) caused by the design behind the chiral proofreading site of D-aminoacyl-tRNA deacylase. PLOS Biol. 14(5) e1002465, 1-22.

  15. Ahmad, S., Muthukumar, S., Kuncha, S. K., Routh, S. B., Yerabham, A., S. K., Hussain, T., Kamarthapu, V., Kruparani, S. P. and Sankaranarayanan, R. (2015) Specificity and catalysis hardwired at the RNA-protein interface in a translational proofreading enzyme. Nature Commun. 6:7552 doi: 10.1038/ncomms8552.

  16. Ahmad, S., Routh, S.B., Kamarthapu, V., Chalissery, J., Muthukumar, S., Hussain, T., Kruparani, S.P., Deshmukh, M.V., and Sankaranarayanan, R. (2013) Mechanism of chiral proofreading during translation of the genetic code, eLife doi: 10.7554/eLife.01519 (highlighted by Science (343) Editor’s Choice, "Chirality check", pp 119, 10th Jan, 2014)

  17. Chhabra, A., Haque, A. S., Pal, R. K., Goyal, A., Rai, R., Joshi, S., Panjikar, S., Pasha, S., Sankaranarayanan, R.* and Gokhale, R. S.* (2012) Nonprocessive [2+2]e- off-loading reductase domains from mycobacterial nonribosomal peptide synthetases.
    Proc. Natl. Acad. Sci. (USA) 109, 5681-5686.

  18. Goyal, A., Verma, P. Anandhakrishnan, M., Gokhale, R. S.* and Sankaranarayanan R.* (2012) Molecular basis of the functional divergence of fatty acyl-AMP ligase biosynthetic enzymes of Mycobacterium tuberculosis. J. Mol. Biol. 416, 221-238.

  19. Hussain, T., Kamarthapu, V., Kruparani, S. P., Deshmukh, M. V. and Sankaranarayanan, R. (2010) Mechanistic insights into cognate substrate discrimination during proofreading in translation. Proc. Natl. Acad. Sci. (USA)107, 22117-22121. (#Covered by an accompanying commentary article in the same PNAS issue titled 'Proofreading in translation: Dynamics of the double-sieve model' p21949-50).

  20. Aparna, G., Chatterjee, A., Sonti, R. V. and Sankaranarayanan, R. (2009) A cell wall degrading esterase of Xanthomonas oryzae requires a unique substrate recognition module for pathogenesis on rice. Plant Cell 21, 1860-1873.

  21. Arora, P., Goyal, A., Natarajan, V. T., Rajakumara, E., Verma, P., Gupta, R., Yousuf, M., Trivedi, O. A., Mohanty, D., Tyagi, A., Sankaranarayanan, R.* and Gokhale, R. S.* (2009) Mechanistic and functional insights into fatty acid activation in
    Mycobacterium tuberculosis.     Nature Chem. Biol. 5, 166-173.

  22. Hussain, T., Kruparani, S. P., Pal, B., Dock-Bregeon, A-C., Dwivedi, S., Shekar, M. R., Sureshbabu, K. and Sankaranarayanan, R. (2006) Posttransfer editing mechanism of a D-aminoacyl-tRNA deacylase-like domain in threonyl-tRNA synthetase from
    archaea.     EMBO J. 25, 4152-4162.

  23. Dwivedi, S., Kruparani, S. P. and Sankaranarayanan, R. (2005) A D-amino acid editing module coupled to the translational apparatus in archaea. Nature Struct. Mol. Biol. 12, 556-557.

  24. Sankaranarayanan, R.*, Saxena, P., Marathe, U., Gokhale, R. S.*, Shanmugam, V. M. and Rukmini, R. (2004) A novel tunnel in mycobacterial type III polyketide synthase reveals the structural basis for generating diverse metabolites. Nature Struct. Mol.
    Biol.     11, 894-900.
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