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RISCI – ‘Repeat Induced Sequence Changes Identifier’ - is a comprehensive, comparative genomics based, in silico subtractive hybridization tool, to identify differential insertions and associated subtle sequence changes like target site duplications (TSD), 3’ and 5' flank transductions, insertion mediated deletions, recombination mediated deletions and polymorphism induced by transposons. Post insertion changes such as recombination mediated deletions and disruptions are also picked up by RISCI. It emulates subtractive hybridization in the sense that, only when the locus in the genomes is differential, does it report the sequence changes associated with the transposon insertion.
RISCI picks up the repeat locus from a given genome (Main genome) and zooms into the orthologous locus in one or more genomes (Comparative genomes) of the same or closely related species and reports whether the insertion is differential or otherwise using a novel comparative genomics based Target site duplication finding strategy. When differential, RISCI also reports additional subtle sequence changes brought about by the transposon insertion in the main genome which may then be studied for their downstream effects. When different genomes of the same species are compared, all identified differential insertions represent polymorphic sites. RISCI also integrates the genomic context (genic or intergenic, if genic- exonic or intronic) of the the repeat locus in the main genome and of the identified orthologous locus in the comparative genome provided the annotation files are available.
DOWNLOADS
RISCI.rar – Perl scripts for RISCI (compatible to LINUX Operating System).
The Readme file for RISCI may be accessed at
http://www.ccmb.res.in/rakeshmishra/tools/RISCI_Readme.htm
For enquiries on RISCI contact vipin@ccmb.res.in |
