< Publications

Our Publications

2015-present

Bakthisaran R, Tangirala R, Rao CM. Small heat shock proteins: Role in cellular functions and pathology. Biochim Biophys Acta. 2015 Apr;1854(4):291-319. doi: 10.1016/j.bbapap.2014.12.019. Epub 2014 Dec 30

Das T, Volety S, Ahsan SM, Thakur AK, Sharma S, Padhi TR, Basu S, Rao CM. Safety, sterility and stability of direct-from-vial multiple dosing intravitreal injection of bevacizumab.Clin Experiment Ophthalmol. 2015 Jul;43(5):466-73. doi: 10.1111/ceo.12489. Epub 2015 Apr 14.

2010-2014

Micheal IJ, Vidyasagar AJ, Bokara KK, Mekala NK, Asthana A, Rao ChM. Foil assisted replica molding for fabrication of microfluidic devices and their application in vitro.Lab Chip. 2014 Oct 7;14(19):3695-9.

Bokara KK, Oggu GS, Vidyasagar AJ, Asthana A, Lee JE, Rao ChM. Modulation of stem cell differentiation by the influence of nanobiomaterials/carriers.Curr Stem Cell Res Ther. 2014;9(6):458-68.

Asthana A, Bollapalli M, Tangirala R, Bakthisaran R, Mohan Rao Ch. Hsp27 suppresses the Cu(2+)-induced amyloidogenicity, redox activity, and cytotoxicity of α-synuclein by metal ion stripping. Free Radic Biol Med. 2014 Jul;72:176-90.

Swamy-Mruthinti S, Srinivas V, Hansen JE, Rao ChM. Thermal Stress Induced Aggregation of Aquaporin 0 (AQP0) and Protection by α-Crystallin via Its Chaperone Function.PLoS One. 2013 Nov 27;8(11):e80404.

Sultan A, Raman B, Rao ChM, Tangirala R. The Extracellular Chaperone Haptoglobin Prevents Serum Fatty Acid-promoted Amyloid Fibril Formation of β2-Microglobulin, Resistance to Lysosomal Degradation, and Cytotoxicity. J Biol Chem. 2013 Nov 8;288(45):32326-42.

Karunakaran SC, Babu PS, Madhuri B, Marydasan B, Paul AK, Nair AS, Rao KS, Srinivasan A, Chandrashekar TK, Rao CM, Pillai R, Ramaiah D. In vitro Demonstration of Apoptosis Mediated Photodynamic Activity and NIR Nucleus Imaging through a Novel Porphyrin. ACS Chem Biol. 2013 Jan 18;8(1):127-32.

Asthana A, Raman B, Ramakrishna T, Rao ChM. Structural aspects and chaperone activity of human HspB3: role of the "C-terminal extension". Cell Biochem Biophys. 2012 Sep;64(1):61-72.

Prabhu S, Raman B, Ramakrishna T, Rao ChM. HspB2/myotonic dystrophy protein kinase binding protein (MKBP) as a novel molecular chaperone: structural and functional aspects. PLoS One. 2012;7(1):e29810. doi: 10.1371/journal.pone.0029810.

Mishra P, Prabha CR, Rao ChM, Volety S. Q2N and S65D substitutions of ubiquitin unravel functional significance of the invariant residues Gln2 and Ser65. Cell Biochem Biophys. 2011 Dec;61(3):619-28.

Thakur AK, Srivastava AK, Srinivas V, Chary KV, Rao CM. Copper alters aggregation behavior of prion protein and induces novel interactions between its N- and C-terminal regions. J Biol Chem. Nov 4;286(44):38533-45.2011.

Prabhu S, Srinivas V, Ramakrishna T, Raman B, Rao ChM. Inhibition of Cu(2+)-mediated generation of reactive oxygen species by the small heat shock protein αB-crystallin: The relative contributions of the N- and C-terminal domains. Free Radic Biol Med. 51(3):755-62; 2011

Zhang Q, Wang D, Singh NK, Kundumani-Sridharan V, Gadiparthi L, Rao ChM, Rao GN. Activation of Cytosolic Phospholipase A2 Downstream of the Src-Phospholipase D1 (PLD1)-Protein Kinase C {gamma} (PKC{gamma}) Signaling Axis Is Required for Hypoxia-induced Pathological Retinal Angiogenesis. J Biol Chem. 286(25):22489-98; 2011

Adhikari AS, Singh BN, Rao KS, Rao ChM. αB-crystallin, a small heat shock protein, modulates NF-kB activity in a phosphorylation-dependant manner and protects muscle myoblasts from TNF-α induced cytotoxicity. Biochim Biophys Acta. 1813(8):1532-42; 2011

Taiyab A., Rao ChM. HSP90 modulates actin dynamics: inhibition of HSP90 leads to decreased cell motility and impairs invasion. Biochim Biophys Acta. 1813(1):213-21, 2011

Singh BN, Rao KS, Rao ChM. Ubiquitin–proteasome-mediated degradation and synthesis of MyoD is modulated by αB-crystallin, a small heat shock protein, during muscle differentiation. Biochim Biophys Acta. 2010 Feb;1803(2):288-99.

2005-2009

D. Singh, T. Ramakrishna, B. Raman, Ch. Mohan Rao. Synergistic effects of metal ion and the pre-senile cataract-causing G98R αA-crystallin: self-aggregation propensities and chaperone activity. Mol. Vis.;15:2050-2060, 2009

P Mishra, S Volety, Ch. Mohan Rao, CR Prabha. Glutamate64 to glycine substitution in G1 beta-bulge of ubiquitin impairs function and stabilizes structure of the protein. Journal of Biochemistry, 146, 563-569, 2009

A Taiyab, AS Sreedhar, Ch. Mohan Rao. Hsp90 inhibitors, GA and 17AAG, lead to ER stress-induced apoptosis in rat histiocytoma. Biochemical Pharmacology, 78, 142-152, 2009

MF Ahmad, D Singh, A Taiyab, T Ramakrishna, B Raman, Ch. Mohan Rao. Selective Cu2+ binding, redox silencing, and cytoprotective effects of the small heat shock proteins alphaA- and alphaB-crystallin. Journal of Molecular Biology, 382, 812-824, 2008

AK Thakur, Ch. Mohan Rao. UV-light exposed prion protein fails to form amyloid fibrils. PLoS One, 3, e2688, 2008

MF Ahmad, B Raman, T Ramakrishna, Ch. Mohan Rao. Effect of phosphorylation on alpha B-crystallin: differences in stability, subunit exchange and chaperone activityof homo and mixed oligomers of alpha B-crystallin and its phosphorylation-mimicking mutant. Journal of Molecular Biology, 375, 1040-1051, 2008

Devendra Singh, B Raman, T Ramakrishna and Ch. Mohan Rao : Mixed oligomer formation between human aA-crystallin and its cataract-causing G98R mutant: Structural stability and functional differences. Journal of Molecular Biology, 373, 1293-1304, 2007

Bhairab N Singh, K Sridhar Rao, T Ramakrishna, Nandini Rangaraj and Ch. Mohan Rao : Association of aB-crystallin, a small heat shock protein, with actin: Role in modulating actin filament dynamics in vivo. Journal of Molecular Biology, 366, 756-767, 2007

Tirumala K Chowdary, B Raman, T Ramakrishna and Ch. Mohan Rao : Interactions of mammalian Hsp22 with lipid membranes. Biochemical Journal, 401, 437-445, 2007

Ch. Mohan Rao, T Ramakrishna, Saloni Y Pasta and B Raman : a-Crystallins, small heat shock proteins with diverse functions in cell survival and stress tolerance. In Stress Response: A Molecular Biology Approach. (Eds: Sreedhar, A. S. ansd Srinivas U. K.) Research Signpost, Trivandrum, India, 2006

Devendra Singh, B Raman, T Ramakrishna and Ch. Mohan Rao : The cataract-causing mutation G98R in human aA-crystallin leads to folding defects and loss of chaperone activity. Molecular Vision, 12, 1372-1379, 2006

Md. Faiz Ahmad, T Ramakrishna, B Raman and Ch. Mohan Rao : Fibillogenic and non-fibrillogenic ensembles of SDS-bound human alpha synuclein. Journal of Molecular Biology, 364, 1061-1072, 2006

B Raman, Eri Chatani, Miho Kihara, Tadato Ban, Miyo Sakai, Kazuhiro Hasegawa, Hironobu Naiki, Ch. Mohan Rao and Yuji Goto : Critical balance of electrostatic and hydrophobic interactions is required for b2-microglobulin amyloid fibril growth and stability. Biochemistry, 44, 1288-1299, 2005

B Raman, Tadato Ban, Miyo Sakai, Saloni Y Pasta, T Ramakrishna, Hironobu Naiki, Yuji Goto and Ch. Mohan Rao : AlphaB- crystallin, a small heat-shock protein, prevents the amyloid fibril growth of an amyloid B-peptide adn B2 microglobulin. Biochemical Journal, 392, 573-581, 2005

V Srinivas, B Raman, K Sridhar Rao, T Ramakrishna and Ch. Mohan Rao :Arginine hydrochloride enhances the dynamics of subunit assembly and the chaperone-like activity of a-crystallin. Molecular Vision, 11, 249-55, 2005

2000-2004

C Ratna Prabha and Ch. Mohan Rao : Oxidative refolding of lysozyme in trifluoroethanol (TFE) and ethylene glycol: interfering role of preexisting a-helical structure and intermolecular hydrophobic interactions. FEBS Letters, 557, 69-72, 2004

Tirumala Kumar Chowdary, B Raman, T. Ramakrishna and Ch. Mohan Rao : Mammalian Hsp22 is a heat-inducible small heat-shock protein with chaperone-like activity. Biochem. J., 381, 379-387, 2004

Saloni Pasta, B.Raman, T. Ramakrishna and Ch. Mohan Rao : The IXI/V Motif in the C-Terminal Extension of a-Crystallins: Alternative Interactions and Oligomeric Assemblies. Molecular Vision, 10, 655-662, 2004

Amit S Adhikari, K Sridhar Rao, Nandini Rangaraj, Veena K Parnaik and Ch. Mohan Rao : Heat stress-induced localization of small heat shock proteins in mouse myoblasts: intranuclear lamin A/C speckles as target for aB-crystallin and Hsp25Oligomeric. Exp. Cell Res., 299, 393-403, 2004

Mohd. Waseem Akhtar, V Srinivas, B Raman, T Ramakrishna, Tomonao Inobe, kosuke Maki, Munehito Arai, Kunihiro Kuwajima and Ch. Mohan Rao : Hsp33 with enhanced chaperone activity. J. Biol. Chem., 279, 55760-55769, 2004

V. Srinivas, B. Raman, K. Sridhar Rao, T.Ramakrishna and Ch.Mohan Rao : Structural perturbation and enhancement of the chaperone-like activity of a-crystallin by arginine hydrochloride. Protein Science,12, 1262-1270, 2003

Saloni Y Pasta, B. Raman, T. Ramakrishna and Ch. Mohan Rao : Role of the conserved SRLFDQFFG region of alpha crystallin, a small heat shock protein: effect on the oligomeric size, subunit exchange and chaperone-like activity. J. Biol. Chem., 278, 51159-51166, 2003

Ranvir Singh and Ch. Mohan Rao : Chaperone-like activity and surface hydrophobicity of 70S ribosome. FEBS Letters, 527, 234-238, 2002

Ch. Mohan Rao, T. Ramakrishna and B. Raman : Alpha-crystallin : A small heat shock protein with chaperone activity. PINSA, 68, 349-365, 2002

Saloni Y Pasta, B. Raman, T. Ramakrishna and Ch. Mohan Rao : Role of the C-terminal extensions of a-crystallins. J. Biol. Chem., 277, 45821-45828, 2002

B. Raman, L.V. Siva Kumar, T. Ramakrishna and Ch. Mohan Rao : Redox-regulated chaperone function and conformational changes of Escherichia coli Hsp33. FEBS Letters, 489, 19-24, 2001

Shradha Goenka and Ch. Mohan Rao : Expression of recombinant z-crystallin in E.coli with the help of GroEL/ES and its purification. Protein Expression and Purification, 21, 260-267, 2001

Usha Gopinathan, T. Ramakrishna, Mark Willcox, Ch. Mohan Rao, D. Balasubramanian, Ajay Kulkarni, Geeta Kashyap Vemuganti, Gullapalli N. Rao : Enzymatic, clinical and histologic evaluation of corneal tissues in experimental fungal keratitis in rabbits. Experimental Eye Research, 72(4), 433-443, 2001

V. Srinivas, Siddhartha A. Datta, T. Ramakrishna and Ch.Mohan Rao : Studies on the a-crystallin target protein binding sites: sequential binding with two target proteins. Molecular Vision,7, 114-119, 2001

K. Rajaraman, B. Raman, T. Ramakrishna and Ch. Mohan Rao : Interaction of human recombinant aA- and aB-crystallins with early and late unfolding intermediates of citrate synthase on its thermal denaturation. FEBS Letters, 497, 118-123, 2001

Shradha Goenka, B. Raman, T. Ramakrishna and Ch. Mohan Rao : Unfolding and refolding of a quinone oxidoreductase: a-crystallin, a molecular chaperone, assists its reactivation. Biochem. J., 359, 547-556, 2001

M.V. Jagannadham, M.K. Chattopadhyay, C. Subbalakshmi, M. Vairamani, K. Narayanan, Ch. Mohan Rao and S. Shivaji : Carotenoids of an Antarctic psychrotolerant bacterium, Sphingobacterium antarcticus and a mesophilic bacterium Sphingobacterium multivorum. Arch. Microbiol., 173, 418-424, 2000

L.V. Siva Kumr and Ch. Mohan Rao : Domain swapping in human aA and aB crystallins affects oligomerization and enhances chaperone-like activity. JBC, 275, 22009-22013, 2000

M. Vithal, B. Srinivasulu, K. Koteswara Rao and Ch. Mohan Rao : Preparation, characterization, ESR and PAS studies of Cu0.5NbAIP3O12 and HNbAIP3O12 . Materials Letters, 45, 58-62, 2000

Shradha Goenka and Ch. Mohan Rao : Inability of chaperones to fold mutant z-crystallin, an aggregation-prone eye lens protein. Molecular Vision, 6, 232-236, 2000

Siddhartha A. Datta and Ch. Mohan Rao : Packing-induced conformational and functional changes in the subunits of a-crystallins. JBC, 275, 41004-41010, 2000

1995-1999

L.V. Siva Kumar, T. Ramakrishna and Ch. Mohan Rao : Structural and functional consequences of the mutation of a Conserved arginine residue in aA and aB crystallins. JBC, 274, 24137-24141, 1999

V.D. Trivedi, B. Raman, T. Ramakrishna and Ch. Mohan Rao : Detection and assay of proteases using calf lens b-crystallin aggregate as substrate. J. Biochem. Biophys. Methods, 40, 49-55, 1999

Siddhartha A. Datta and Ch. Mohan Rao : Differential temperature-dependent chaperone-like activity aA- and aB-crystallin homoaggregates. JBC, 274, 34773-34778, 1999

Jose Jimenez-Asensio, Christine Colvis, Jeff Kowalak, Arireza Janjani, Yvonne Duglas-Tabor, Maria Moroni, Umberto Mura, Ch. Mohan Rao, D. Balasubramanian, Manuel B. Datiles and Donita Garland : An atypical form of aB-crystallin is present in high concentration in some human cataractous lenses: identification and characterization of aberrant N- and C-terminal processing. JBC, 274, 32287-32294, 1999

Ch. Mohan Rao,B. Raman,T. Ramakrishna, K. Rajaraman, D. Ghosh, S. Datta, V.D. Trivedi and M.B. Sukhaswami : Structural perturbation of a-crystallin and its chaperone-like activity. IJBM, 22, 271-281, 1998

K. Rajaraman, B. Raman, T. Ramakrishna and Ch. Mohan Rao : The chaperone-like a-crystallin forms a complex only with the aggregation-prone molten globule state of a-lactalbumin. BBRC, 249, 971-921, 1998

K Narayanan, S Chandani, T Ramakrishna and Ch. Mohan Rao : Depth profiling of mammalian cells by photoacoustic spectroscopy: localization of ligands. Biophys. J., 72, 2365-2368, 1997

B. Raman and Ch. Mohan Rao : Chaperone-like activity and temperature-induced structural changes of a-crystallin. J. Biol. Chem., 272, 23559-23564, 1997

B. Raman, T.Ramakrishna and Ch. Mohan Rao : Effect of chaperone-like alpha-crystallin on the refolding of lysozyme and ribonuclease A. FEBS Letters,416, 369-372, 1997

V.D. Trivedi, B. Raman, Ch. Mohan Rao and T.Ramakrishna : Co-refolding denatured-reduced hen egg white lysozyme with acidic and basic proteins. FEBS Letters, 418, 363-366, 1997

B Raman, T Ramakrishna and Ch. Mohan Rao : Refolding of denatured and denatured-reduced lysozyme at high concentrations. J. Biol. Chem., 271, 17067-17072, 1996

M V Jagannadham, K Narayanan and Ch. Mohan Rao : In vivo characteristics and localisation of carotenoid pigments in psychrotrophic and mesophilic Micrococcus roseus using photoacoustic spectroscopy. BBRC, 227, 221-226, 1996

K Rajaraman, B Raman and Ch. Mohan Rao : Molten-globule state of carbonic anhydrase binds to the chaperone-like a-crystallin. J. Biol. Chem., 271, 27595-27600, 1996

T Suresh Kumar, B Raman and Ch. Mohan Rao : Fluorescent staining for proteins on poly-acrylamide gels with 5-dimethylamino-1-naphthalenesulfonyl chloride (Dansyl Chloride). J. Biochem. Biophys. Methods, 30, 79-84, 1995

Ch. Mohan Rao, G Chuan Quin, W G Robinson and S Zigler : Effect of smoke condensate on the physiological integrity and morphology or organ cultured rat lenses. Current Eye Research,14, 295-301, 1995

B Raman, T Ramakrishna and Ch. Mohan Rao : The temperature dependent chaperone-like activity of alpha-crystallin. FEBS Letts.,365, 133-136, 1995

B Raman, T Ramakrishna and Ch. Mohan Rao : Rapid refolding studies on the chaperone-like alpha-crystallin. J. Biol. Chem., 270, 19888-19892, 1995

1990-1994

Rao, Ch. Mohan : Eye lens and its protein. Biobytes, 3, 13-16, 1994

Rao, S C and Rao, Ch. Mohan : Red edge excitation shifts of crstallins and intact eye lenses: a study of fragmental mobility and interprotein interactions. FEBS Letts., 337, 269-273, 1994

Ch. Mohan Rao and J. Samuel Zigler, Jr. : On the intracellular stability of lens crystallins. Exp. Eye Res., 58, 127-128, 1994 (Letter to the Editors)

B. Raman and Ch. Mohan Rao : Chaperone like activity and quaternary structure of alpha-crystallin. J. Biol. Chem., 269, 27264-27268, 1994

Rao, Ch. Mohan and Zigler, J S. : Are crystallins designed for high intracellular stability? Exp.Eye Res.,56, 615-619, 1993

D Balasubramanian, A K Bansal, S Basti, K S Bhat, J S Murthy and Ch. Mohan Rao :The biology of cataract. Indian J. Ophthalmology, 41, 153-171, 1993

Rao, Ch. Mohan and Zigler, J S. : Levels of reduced nucleotides alter photochemical damage to intact lenses. Photochem. and Photobiol., 56, 523-528, 1992

Rao, Ch. Mohan : Synchronous scan fluorescence spectroscopy of proteins and human eye lenses. Biochem.Biophys. Res. Commun., 176, 1351-1357, 1991

Reddy, G, Reddy, K N, Reddy, M and Rao, Ch. Mohan : On photoacoustic spectra of X-irradiated NaCl: Y crystalls. Physics Letters A., 156, 253-255, 1991

S. Chenchal Rao, Ch. Mohan Rao and D. Balasubramanian : The conformational status of a protein influences the aerobid photolysis of its tryptophan residues. Photochem. and Photobiol., 51, 357-362, 1990

Srinivas, V and Rao, Ch. Mohan : Time profile of hemin aggregation: An analysis. Biochem.Int., 21,849-855, 1990

Rao, Ch. Mohan : Effect of ultraviolet radiation on intact eye lens. Bulletin of Radiation Protection, 13(2),1990

1985-1989

Rao, Ch. Mohan, Chenchal S. Rao and Rao, P. Bhima : Red edge excitation effect in intact eye lens. Photochemistry and Photobiol., 50, 399-402, 1989

Sharma Y, Rao, Ch. Mohan, Narasu M. Laxmi, Rao S Chenchal, Gopalakrishna, A and Balasubramanian, D. : Calcium ion bind to d- and to b-cyrstallins: The presence of the EF-HAND motif in crystallin that aids in calcium ion binding. J. Biol. Chem., 264, 12794-12799, 1989

Sharma Y, Ch. Mohan Rao, S. Chenchal, Gopalakrishna, A, Somasundaram, T and Balasubramanian, D. Binding site conformation rules the color of the dye Stains-all: A study with eye lens proteins. J.Biol.Chem. 264, 20923-20927, 1989

Rao, Ch. Mohan, Balasubramanian, D and Chakrabarti, B. : Monitoring light-induced changes in isolated intact eye lenses. Photochem. Photobiol., 46, 511-151, 1987

Rao, Ch. Mohan : Photoacoustic spectroscopy - Instrumentation. J. Instruments Society of India, 17, 219-227, 1987

Balasubramanian, D, Ch. Mohan and Kshirsagar, S T. : Raman spectroscopic analysis of surfactants in the neat, monomeric and micellar phases. Springer Verlag, 247-250, 1987

Balasubramanian, D and Rao, Ch. Mohan : Application of photoacoustics of biology - some specific systems and methods. Canadian J. Physics, 64, 1132-1135, 1986

1980-1984

Balasubramanian, D, Rao, Ch. Mohan and Panijpan, B. : The malaria parasite monitored by photoacoustic spectroscopy. Science, 223, 828-830, 1984

Panijpan, B.,Ch. Mohan Rao and Balasubramanian, D. : Interaction of antimalarial drugs with hemin. Bioscience Reports, 3, 1113-1117, 1983

Balasubramanian, D and Rao, Ch. Mohan : Biological applications of photoacoustic spectroscopy .Current Science, 51, 111-117, 1982

Rao, Ch. Mohan and Balasubramanian, D. : Study of a solid state reaction by photoacoustic spectroscopy. Physical Chemistry, 86, 939-943, 1982

Balasubramanian, D and Rao, Ch. Mohan : Photoacoustic spectroscopy of biological systems. Photochemistry and Photobiology, 34, 749-752, 1981

Rao Ch. Mohan and Balasubramanian, D. : Investigation of acidity of catalyst surfaces by photoacoustic spectroscopy. Spectroscopy Letters, 13, 329-337, 1980